Silica-coated natural iron ore particles for genomic DNA separation

Sarangerel Oidovsambuu; Tuul Tsagaantsooj; Ganzorig Chimed; Ochirkhuyag Bayanjargal; Enkhtsetseg Dashjav; Sansarmaa Sanchigdorj; Odgerel Oidovsambuu; Ninjbadgar Tsedev

doi:10.5564/mjc.v26i54.4144

Authors

Sarangerel Oidovsambuu Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences, Ulaanbaatar 13330, Mongolia https://orcid.org/0000-0001-5062-7440
Tuul Tsagaantsooj School of Engineering and Technology, National University of Mongolia, Ulaanbaatar 14201, Mongolia https://orcid.org/0000-0003-2064-9205
Ganzorig Chimed School of Engineering and Technology, National University of Mongolia, Ulaanbaatar 14201, Mongolia https://orcid.org/0009-0007-4042-6814
Ochirkhuyag Bayanjargal School of Engineering and Technology, National University of Mongolia, Ulaanbaatar 14201, Mongolia https://orcid.org/0000-0003-0640-3313
Enkhtsetseg Dashjav Forschungszentrum Jülich GmbH, Institute of Climate and Energy Systems (IEK-1), Jülich 52425, Germany https://orcid.org/0000-0002-7823-7759
Sansarmaa Sanchigdorj School of Engineering and Technology, National University of Mongolia, Ulaanbaatar 14201, Mongolia https://orcid.org/0009-0000-7433-4503
Odgerel Oidovsambuu School of Engineering and Technology, National University of Mongolia, Ulaanbaatar 14201, Mongolia https://orcid.org/0000-0001-6872-3385
Ninjbadgar Tsedev Institute of Food Research, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia. https://orcid.org/0000-0002-5725-3637

DOI:

https://doi.org/10.5564/mjc.v26i54.4144

Keywords:

Magnetic, , natural ore,, silica coating,, genomic DNA separation

Abstract

As an alternative way to separation of biological molecules, silica coated magnetite ore particles were prepared through two step synthetic method including ball milling of iron ore powder followed by silica coating with Stöber method. As synthesized silica coated magnetic ore particles were composed mainly of magnetite and other accompanying minerals such as hematite and Al₂O₃, ZrO₂. The particles were irregular in shape, with average size of 0.4 ± 0.3 µm as demonstrated with SEM. Field dependent magnetization showed that silica-coated magnetite ore particles are soft ferromagnet with coercivity of 320Oe and remanent magnetization of 2.26 emu/g. Using these particles, genomic DNA was successfully separated from E. coli with sufficient yield and purity comparable to those obtained with a commercial magnetic separation kit, demonstrating their potential for bioseparation from diverse biological sources.

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