Phylogenetic analysis of mitochondrial cox-1 gene region as a DNA barcode marker for the Gray wolf, Canis lupus in Mongolia
DOI:
https://doi.org/10.5564/pmas.v65i03.4607Keywords:
Saaral chono, Canis lupus, DNA barcoding, illegal hunting, Near threatened speciesAbstract
The gray wolf (Canis lupus), locally known as saaral chono, is a widely distributed species across Europe, North America, and Asia, including Mongolia. Over the last 45 years, Mongolia’s gray wolf population has declined threefold, highlighting the need for conservation measures. This study investigates the utility of the mitochondrial cytochrome c oxidase subunit 1 (cox-1) gene as a DNA barcode marker for gray wolves in Mongolia. Tissue samples from ten individuals across ten provinces of Mongolia were collected and sequenced for a region of the cox-1 gene. Phylogenetic analyses show that Mongolian gray wolves cluster with other C. lupus sequences from GenBank, forming two distinct subclades within the species. The cox-1 sequences clearly differentiate gray wolves from other Mongolian Canidae species, demonstrating their value for species identification. These findings support the use of cox-1 as a reliable DNA barcode for monitoring, forensic investigations of illegal hunting, and broader conservation efforts.
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1. P. Kaczensky, N. Enkhsaikhan, O. Ganbaatar, and C. Walzer, “The great Gobi b strictly protected area in Mongolia - refuge or sink for wolves cards lupus in the Gobi?,” Wildlife Biol., vol. 14, no. 4, pp. 444–456, 2008, https://doi.org/10.2981/0909-6396-14.4.444.
2. J. C. Avise, Phylogeography. Harvard University Press, 2000. doi: 10.2307/j. ctv1nzfgj7.
3. P. D. N. Hebert, A. Cywinska, S. L. Ball, and J. R. DeWaard, “Biological identifications through DNA barcodes,” Proc. R. Soc. B Biol. Sci., vol. 270, no. 1512, pp. 313–321, 2003, https://doi.org/10.1098/rspb.2002.2218.
4. M. Bayarlkhagva, B. Ulziibat, B. Gun-Aajav, D. Bazarsad, B. Damdingiin, and E. Batmagnai, “Phylogenetic analysis of a region of mitochondrial cox-1 as a DNA barcode marker sequence of Gazella subgutturosa (goitered gazelle) in Mongolia,” Anim. Biodivers. Conserv., vol. 46, no. 2, pp. 213–217, 2023, https://doi.org/10.32800/abc.2023.46.0213.
5. M. Bayarlkhagva and E. Batmagnai, “Phylogenetic analysis of a region of mitochondrial cox-1 as a DNA barcode marker sequence for the Siberian ibex Capra sibirica ( Bovidae:,” vol. 6, no. 2, pp. 32–37, 2024.
6. M. S. Rodrigues, K. A. Morelli, and A. M. Jansen, “Cytochrome c oxidase subunit 1 gene as a DNA barcode for discriminating Trypanosoma cruzi DTUs and closely related species,” Parasites and Vectors, vol. 10, no. 1, pp. 1–18, 2017, https://doi.org/10.1186/s13071-017-2457-1.
7. A. V. Ivanova, N. V., Clare, E. L. & Borisenko, “An inexpensive, automation-friendly protocol for recovering high-quality DNA,” Methods Mol. Biol. DNA barcoding mammals., pp. 153–182, 2012, https://doi.org/10.1007/978-1-61779-591-6.
8. S. Kumar, G. Stecher, M. Li, C. Knyaz, and K. Tamura, “MEGA X: Molecular evolutionary genetics analysis across computing platforms,” Mol. Biol. Evol., vol. 35, no. 6, pp. 1547–1549, 2018, https://doi.org/10.1093/molbev/msy096.
9. M. Hasegawa, H. Kishino, and T. Aki Yano, “Dating of the human-ape splitting by a molecular clock of mitochondrial DNA,” J. Mol. Evol., vol. 22, no. 2, pp. 160–174, 1985, https://doi.org/10.1007/BF02101694.
10. J. Felsenstein, “Confidence Limits on Phylogenies: an Approach Using the Bootstrap,” Evolution (N.Y)., vol. 39, no. 4, pp. 783–791, 1985, https://doi.org/ 10.1111/j.1558-5646.1985.tb00420.x.
11. S. Koblmüller et al., “Whole mitochondrial genomes illuminate ancient intercontinental dispersals of grey wolves (Canis lupus),” J. Biogeogr., vol. 43, no. 9, pp. 1728–1738, 2016, https://doi.org/10.1111/jbi.12765.
12. L. Loog et al., “Ancient DNA suggests modern wolves trace their origin to a Late Pleistocene expansion from Beringia,” Mol. Ecol., vol. 29, no. 9, pp. 1596–1610, 2020, https://doi.org/10.1111/mec.15329.
13. X. Wang et al., “Ancient DNA reveals the origin and history of early dogs in northeastern China,” J. Archaeol. Sci., vol. 168, p. 106010, 2024, https://doi.org/10.1016/j.jas.2024.106010.
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Copyright (c) 2025 Bayarlkhagva Damdin, Bolortuya Ulziibat, Bayarmaa Gun-Aajav, Davaa Bazarsad, Oyuntsetseg Dashzeveg, Enkhbaatar Batmagnai
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