Preparation of “Open/closed” pores of PLGA-microsphere for controlled release of protein drug

  • Odonchimeg M Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences, MAS 4th building, Peace Avenue, Bayanzurkh district, Ulaanbaatar 13330, Mongolia
  • S C Kim School of Nano Engineering, Inje University, Gimhae 621-749, Korea
  • Y K Shim School of Nano Engineering, Inje University, Gimhae 621-749, Korea
  • W K Lee School of Nano Engineering, Inje University, Gimhae 621-749, Korea
Keywords: porous microspheres, lysozyme, PLGA,

Abstract

Poly(D,L-lactic-co-glycolic acid)  has been extensively used as a controlled release carrier for drug delivery due to its good biocompatibility, biodegradability, and mechanical strength. In this study, porous PLGA microspheres were fabricated by an emulsion-solvent evaporation technique using poly ethylene glycol (PEG) as an extractable porogen and loaded with  protein (lysozyme) by suspending them in protein solution. For controlled release of protein, porous microspheres containing lysozyme were treated with water-miscible solvents in aqueous phase for production of pore-closed microspheres. The surface morphology of microspheres were investigated using scanning electron microscopy (SEM) for confirmation of its porous microstructure structure. Protein property after release was observed by enzymatic activity assay. The pore-closing process resulted in nonporous microspheres which exhibited sustained release patterns over an extended period.

Downloads

Download data is not yet available.
Abstract
194
PDF
210
Published
2018-02-13
How to Cite
M, O., Kim, S., Shim, Y., & Lee, W. (2018). Preparation of “Open/closed” pores of PLGA-microsphere for controlled release of protein drug. Mongolian Journal of Chemistry, 18(44), 41-47. https://doi.org/10.5564/mjc.v18i44.936
Section
Articles