Antibacterial and photocatalytic effects of newly synthesized zinc oxide nanoparticles derived from Mongolian honey


  • Anujin Lkhagvadorj Department of Molecular Biology and Genetics, School of Bio-Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, Mongolia
  • Tserendolgor Batsukh Department of Pharmacy Administration and Technology, Mongolian University of Pharmaceutical Sciences, Ulaanbaatar 18130, Mongolia
  • Altansukh Tsend-Ayush Department of Molecular Biology and Genetics, School of Bio-Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, Mongolia



Antibacterial effect;, photocatalytic effect, synthesis, nanoparticle, zinc


Development of bio-compatible, bio-safe and environmentally friendly nanoparticles is a matter of urgency for research in the field of nanotechnology. In this study, we aimed to prepare zinc oxide nanoparticles from Mongolian honey as raw material and to determine its biological activities. Honey-based zinc oxide nanoparticles were obtained by green synthesis method, and their characteristics and biological activities were evaluated. Developed zinc oxide nanoparticles from Khentii honey and Selenge honey were at a size of 16.02 nm and 95.23 nm, respectively. A characteristic band of Khentii honey-based zinc oxide nanoparticles was observed at 466 cm–1 and a band of Selenge honey-based zinc oxide nanoparticles was also observed at 434 cm–1. Antibacterial and photocatalytic effects were detected for the developed nanoparticles. The study suggested that newly synthesized honey-based zinc oxide nanoparticles might be an effective tool against bacterial infection.


Download data is not yet available.


Dhandapani K.V., Anbumani D., Gandhi A.D., Annamalai P., Muthuvenkatachalam B.S., et al. (2020) Green route for the synthesis of zinc oxide nanoparticles from Melia azedarach leaf extract and evaluation of their antioxidant and antibacterial activities. Biocatalysis and Agricultural Biotechnology, 24, 101517.

Siddiqi K.S., ur Rahman A., Tajuddin N., Husen A. (2018) Properties of zinc oxide nanoparticles and their activity against microbes. Nanoscale Res. Lett., 13, 1-13.

Heideman G., Noordermeer J.W., Datta R.N., van Baarle B., et al. (2006) Various ways to reduce zinc oxide levels in S‐SBR rubber compounds. Macromol. Symp., 245-246(1), 657-667.

Husen A. (2017) Gold nanoparticles from plant system: synthesis, characterization and their application. Nanoscience and plant-soil systems, 17, 455-79.

Deka B., Baruah C., Babu A., Kalita P. (2022) Biological and non-conventional synthesis of zinc oxide nanoparticles (ZnO-NPs): Their potential applications. J. Nanotech. and Nanomat., 3(2), 79-89.

Frederickson C.J., Koh J.Y., Bush A.I. (2005) The neurobiology of zinc in health and disease. Nat. Rev. Neurosci., 6(6), 449-62.

Sharmila M., Jothi Mani R., Kader A., Ahmad A., Eldesoky G.E., et al. (2021) Photocatalytic and biological activity of ZnO nanoparticles using honey. Coatings, 11(9), 1046.

Hoseini S.J., Darroudi M., Oskuee R.K., Gholami L., Zak A.K. (2015) Honey-based synthesis of ZnO nanopowders and their cytotoxicity effects. Adv. Powder Tech., 26(3), 991-6.

Porcza L.M., Simms C., Chopra M. (2016) Honey and cancer: current status and future directions. Diseases, 4(4), 30.

Sun L.P., Shi F.F., Zhang W.W., Zhang Z.H., Wang K. (2020) Antioxidant and anti-inflammatory activities of safflower (Carthamus tinctorius L.) honey extract. Foods, 9(8), 1039.

Tsevegmid K., Dooshin S., Ramsey S., Chantawannakul P. (2018) Beekeeping in Mongolia. In: Chantawannakul, P., Williams, G., Neumann, P. (eds) Asian Beekeeping in the 21st Century. Springer, Singapore, 199-221.

Kim Y.S. (2018) Introduction to beekeeping and melliferous plants in Mongolia. Journal of Apiculture, 33(1), 55-62.

Mollaeva M.R., Yabbarov N., Sokol M., Chirkina M., Mollaev M.D., et al. (2021) Optimization, characterization and pharmacokinetic study of meso-tetraphenylporphyrin metal complex-loaded PLGA nanoparticles. Int. J. Mol. Sci., 22(22), 12261.

Ankamwar B.G., Kamble V.B., Annsi J.I., Sarma L.S., Mahajan C.M. (2017) Solar photocatalytic degradation of methylene blue by zno nanoparticles. J. Nanosci. Nanotech., 17(2), 1185-1192.

Jianrong C., Yuqing M., Nongyue H., Xiaohua W., Sijiao L. (2004) Nanotechnology and biosensors. Biotechnology Advances, 22(7), 505-518.

Hsu J.F., Chu S.M., Liao C.C., Wang C.J., Wang Y.S., et al. (2021) Nanotechnology and nanocarrier-based drug delivery as the potential therapeutic strategy for glioblastoma multiforme: An update. Cancers, 13(2), 195.

Jain A.S., Pawar P.S., Sarkar A., Junnuthula V., Dyawanapelly S. (2021) Bionanofactories for green synthesis of silver nanoparticles: Toward antimicrobial applications. Int. J. Mol. Sci., 22(21), Available from:

Santhoshkumar J., Kumar S.V., Rajeshkumar S. (2017) Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resource-Efficient Technologies, 3(4), 459-465.

Sohail M.F., Rehman M., Hussain S.Z., Huma Z.E., Shahnaz G., et al. (2020) Green synthesis of zinc oxide nanoparticles by Neem extract as multi-facet therapeutic agents. Journal of Drug Delivery Science and Technology, 59, 101911.

Bahari N., Hashim N., Abdan K., Md Akim A., Maringgal B., et al. (2023) Role of honey as a bifunctional reducing and capping/stabilizing agent: Application for silver and zinc oxide nanoparticles. Nanomaterials (Basel, Switzerland), 13(7), 1244.

Balasooriya E.R., Jayasinghe C.D., Jayawardena U.A., Ruwanthika R.W.D., Mendis de Silva R., et al. (2017) Honey mediated green synthesis of nanoparticles: New era of safe nanotechnology. Journal of Nanomaterials, 2017, 5919836.

Ifeanyichukwu U.L., Fayemi O.E., Ateba C.N. (2020) Green synthesis of zinc oxide nanoparticles from pomegranate (Punica granatum) extracts and characterization of their antibacterial activity. Molecules (Basel, Switzerland), 25(19), 5919836.

Sarkhosh S., Kahrizi D., Darvishi E., Tourang M., Haghighi-Mood S., et al. (2022) Effect of zinc oxide nanoparticles (ZnO-NPs) on seed germination characteristics in two brassicaceae family species: Camelina sativa and Brassica napus L. Journal of Nanomaterials, 2022, 1892759.

Vijayakumar N., Bhuvaneshwari V.K., Ayyadurai G.K., Jayaprakash R., Gopinath K., et al. (2022) Green synthesis of zinc oxide nanoparticles using Anoectochilus elatus, and their biomedical applications. Saudi Journal of Biological Sciences, 29(4), 2270-9.

Sirelkhatim A., Mahmud S., Seeni A., Kaus N.H.M., Ann L.C., et al. (2015) Review on zinc oxide nanoparticles: Antibacterial activity and toxicity mechanism. Nano-micro Letters, 7(3), 219-42.

Emami-Karvani Z., Chehrazi P. (2011) Antibacterial activity of ZnO nanoparticle on gram-positive and gram-negative bacteria. Afr. J. Microbiol. Res., 5(12), 1368-73.

Behravan M., Hossein Panahi A., Ghizadeh A., Ziaee M., Mahdavi R., et al. (2018) Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int. J. Biol. Macromol., 124, 148-54.

Singh R., Cheng S., Singh S. (2020) Oxidative stress-mediated genotoxic effect of zinc oxide nanoparticles on Deinococcus radiodurans. 3 Biotech., 10(2), 66.

Tahir M.B., Sohaib M., Sagir M., Rafique M. (2022) Role of Nanotechnology in Photocatalysis: Encyclopedia of Smart Materials, 578-89.

Alam M. (2021) Photocatalytic activity of biogenic zinc oxide nanoparticles: In vitro antimicrobial, biocompatibility, and molecular docking studies. Nanotechnology Reviews, 10(1), 1079-91.

Rajapriya M., Sharmili S.A., Baskar R., Balaji R., Alharbi N.S, et al. (2020) Correction to: Synthesis and characterization of zinc oxide nanoparticles using Cynara scolymus leaves: Enhanced hemolytic, antimicrobial, antiproliferative, and photocatalytic activity. Journal of Cluster Science, 31, 791-801.

Das D, Nath BC, Phukon P, Kalita A, Dolui SK. (2013) Synthesis of ZnO nanoparticles and evaluation of antioxidant and cytotoxic activity. Colloids and surfaces B, Biointerfaces, 111, 556-60.

Vinotha V., Iswarya A., Thaya R., Govindarajan M., Alharbi N.S, et al. (2019) Synthesis of ZnO nanoparticles using insulin-rich leaf extract: Anti-diabetic, antibiofilm and anti-oxidant properties. Journal of photochemistry and photobiology B, Biology, 197, 111541.




How to Cite

Lkhagvadorj, A., Batsukh, T., & Tsend-Ayush, A. (2024). Antibacterial and photocatalytic effects of newly synthesized zinc oxide nanoparticles derived from Mongolian honey. Mongolian Journal of Chemistry, 25(51), 35–44.