PLANT REGENERATION OF KAMCHATIC PLANTAIN (PLANTAGO CAMTSCHATICA LINK) THROUGH CALLUS INDUCTION

Authors

  • Ay N.V. School of Animal Sciences and Biotechnology, MULS and College of Agriculture & Applied Biology, Can Tho University
  • Duy M.V. College of Agriculture & Applied Biology, Can Tho University
  • Baatartsogt O. School of Animal Sciences and Biotechnology, MULS
  • Altantsetseg Kh. School of Animal Sciences and Biotechnology, MULS
  • Enkhchimeg V. School of Animal Sciences and Biotechnology, MULS

DOI:

https://doi.org/10.5564/mjas.v19i3.734

Keywords:

Plantago camtschatica Link, plant growth regulators, in vitro culture, organogenesis, plant regeneration, acclimatization

Abstract

In vitro seedling offspring of Plantago camtschatica Link was investigated regarding induction of somatic embryogenesis in petiole/leaf explants from shoot tissue and shoot proliferation. The aim of study was to investigate the medium supplemented with suitable concentration of plant growth regulators in order to induce somatic embryogenesis, plant regeneration and shoot multiplication. The results showed that: (i) Petiole/young leaf of immature stem induced the highest ratio of calli induction and compact calli formation on MS medium supplemented with 1 mgL-1 2,4-D and 0.5 mgL-1 BA; (ii) From created calli, somatic embryogenesis could be induced on MS medium supplemented with 1 mgL-1 TDZ or 1 mgL-1 TDZ and 0.5 mgL-1 NAA; (iii) MS medium supplemented with 5-7 mgL-1 BA shown the most effective on shoot development stage; (iv) Rooting of shoot was the best on 1/2 solid MS medium with activated charcoal (2 gL-1), and 0.5-4 mgL-1 NAA; and (v) acclimatization of micropropagated plants could be planted in plastic pots containing a mixture of decayed straw : rice husk ashes, (1:1, v/v), sand : soil (1:1, v/v) or soil, showed a high survival rate and most seedlings grew normally.

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Published

2017-01-10

How to Cite

N.V., A., M.V., D., O., B., Kh., A., & V., E. (2017). PLANT REGENERATION OF KAMCHATIC PLANTAIN (PLANTAGO CAMTSCHATICA LINK) THROUGH CALLUS INDUCTION. Mongolian Journal of Agricultural Sciences, 19(3), 41–48. https://doi.org/10.5564/mjas.v19i3.734

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