INVESTIGATION ON BOVINE VIRAL DIARRHEA VIRUS AND INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS IN MONGOLIAN DAIRY FARMS
Keywords:BVDV, IBR, ELISA, One-Step RT-PCR, Phylogenetic analysis, BVDV-1
In this study, 168 blood sera were collected from dairy cows in Selenge and Tuv aimags during 2013 - 2014. The ELISA was carried out for serological detection of antibodies and antigens to Bovine viral diarrhea virus (BVDV), antibodies to Infectious bovine rhinotracheitis virus (IBRV) in dairy cows in Mongolia. The ELISA results of antibodies of BVDV and antigen of BVDV showed that 86.9% and 3.57%, respectively. The seroprevalence of antibodies against IBRV was found to be 60.7%. In order to confirm of BVDV, One Step RT-PCR was performed in ELISA positive cattle serum samples using specific primer for BVDV. The results showed that 294 bp fragment was successfully amplified. Phylogenetic analysis revealed that the nucleotide sequences 5'UTR gene of the isolates belonged to the BVDV1 subtype. Four isolated virus samples were closely related to China, the another isolate was closely related to the Slovenia BVDV1 isolate.
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