Optimization and validation of an ELISA using recombinant Toxoplasma gondii matrix antigen 1 for serodiagnosis of the infection

Authors

  • Buyannemekh Tumurjav Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan and Institute of Veterinary Medicine, MULS, Mongolia
  • Mohamad Alaa Terkawi Obihiro University of Agriculture and Veterinary Medicine, Obihiro
  • Houshuang Zhang Obihiro University of Agriculture and Veterinary Medicine, Obihiro
  • Guohong Zhang Obihiro University of Agriculture and Veterinary Medicine, Obihiro
  • Honglin Jia Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan and Research Center for Zoonosis Control, Hokkaido University, Sapporo
  • Junya Yamagishi Obihiro University of Agriculture and Veterinary Medicine, Obihiro
  • Yoshifumi Nishikawa Obihiro University of Agriculture and Veterinary Medicine, Obihiro
  • Ikuo Igarashi Obihiro University of Agriculture and Veterinary Medicine, Obihiro
  • Chihiro Sugimoto Research Center for Zoonosis Control, Hokkaido University, Sapporo
  • Xuenan Xuan Obihiro University of Agriculture and Veterinary Medicine, Obihiro

DOI:

https://doi.org/10.5564/mjas.v15i2.546

Abstract

Toxoplasma gondii infection can be diagnosed directly by polymerase chain reaction (PCR), hybridization and isolation of parasites and indirectly with serological methods[4; 5; 18].Although all these tests have shortcomings, serological tests, particularly the enzyme-linked immunosorbent assay (ELISA), seem to be the most practical and economical. The crude antigen prepared from tachyzoites has been traditionally utilized for commercially serological detection kits. However the use of recombinant antigens can be alternative sources of antigens allowing better standardization of the tests and reducing the costs of production requires mass production of the parasite either from the peritoneal fluids of infected mice or from tissue cultures. In spite of the potential advantages of using recombinant antigens in serology tests, their sensitivities have not yet achieved perfect result; therefore, further research on new antigensis extremely desirable [10; 16; 17; 3]. In this context, the Toxoplasma gondii matrix antigen 1 (TgMAG1) known as 65-kDa protein abundantly expressed within the cyst and in the cyst wall surrounding the bradyzoites [15], has documented to be immunogenic during the infection with T. gondii in mouse model and promising reagent for serodiagnosis of toxoplasmosis in humans [15;12; 6]. However, its usefulness has not yet been confirmed in animal toxoplasmosis.

In this study, the optimization and validation of E.coli-expressed rTgMAG1as ELISA antigen were described

Mongolian Journal of Agricultural Sciences Vol.15(2) 2015; 56-60

Downloads

Download data is not yet available.
Abstract
1683
PDF 1646

Downloads

Published

2015-09-30

How to Cite

Tumurjav, B., Terkawi, M. A., Zhang, H., Zhang, G., Jia, H., Yamagishi, J., Nishikawa, Y., Igarashi, I., Sugimoto, C., & Xuan, X. (2015). Optimization and validation of an ELISA using recombinant Toxoplasma gondii matrix antigen 1 for serodiagnosis of the infection. Mongolian Journal of Agricultural Sciences, 15(2), 56–60. https://doi.org/10.5564/mjas.v15i2.546

Issue

Section

Articles