Comparative study of technology and results of immunoglobulin extraction from placental blood serum and donor blood plasma

Authors

  • Janchivmaa Dorjbat Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia https://orcid.org/0000-0003-2568-6929
  • Bayasgalan Sanchimeg Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Nergui Zoljargal Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Batchuluun Enkhdelger Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Bayaraa Nomin Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Gantulga Anujin Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Davaadulam Nandintsetseg Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Erdenechimeg Uranzaya Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Batmunkh Zolboo Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Amarbayasgalan Altantuul Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Enkhbat Yesukhei Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia
  • Bonduush Ichinkhorloo Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia

DOI:

https://doi.org/10.5564/bicct.v10i10.2602

Keywords:

biopreparation, therapeutic proteins, antibodies, medical biotechnology, gammaglobulin, immunity

Abstract

From 1981 to 2010, protein preparations were produced from maternal placental blood serum at the biopreparation
factory. Starting from 2021, we will continue our production in line with international standards by updating our production
technology and raw materials. In this study, the records, analysis and process of the immunoglobulin extracted from maternal
placental blood serum from 1981-2010 and the production records of the 3-time series of immunoglobulins extracted from donor
blood plasma prepared at the National Transfusion Research Center in 2021 were used. In these, comparative calculations were made
for raw materials, product yield, purity, and consumption of ethanol. As a result of the study, the average yield of immunoglobulin
precipitate separated from placental blood serum was 14.8 ± 2.1 g/l, the purity was 97.9 ± 1.0%, and the amount of spent alcohol was
131.3 ± 6.3 l. However, the average yield of immunoglobulin precipitate separated from the blood plasma of the donor was 25.7 ±
2.4 g/l, the purity was 98.4 ± 0.8%, and the amount of spent alcohol was 96.9 ± 0.7 l. In conclusion, the update of raw materials and
technology of immunoglobulin production is the important for product output, quality and safety.

Амаржигчдын ихсийн цусны ийлдэс болон донорын цусны сийвэнгээс иммуноглобулин ялган авах технологи, үр дүнгийн харьцуулсан судалгаа

Хураангуй: Биобэлдмэлийн үйлдвэрт 1981-2010 оныг хүртэл амаржигчдын ихсийн цусны ийлдсээс уургийн бэлдмэлүүд үйлдвэрлэж байсан бөгөөд 2021 оноос олон улсын жишгийн дагуу үйлдвэрлэлийн технологи, түүхий эдэд шинэчлэл хийн үйл ажиллагааг үргэлжлүүлж байна. 1981-2010 оныг хүртэл Амаржигчдын ихсийн цусны ийлдсээс Иммуноглобулин үйлдвэрлэсэн болон шинжилсэн, зарцуулсан бүртгэл, Цус сэлбэлт судлалын үндэсний төвд бэлтгэсэн донорын цусны сийвэнгээс 2021 онд үйлдвэрлэсэн Иммуноглобулины 3 удаагийн цувралын үйлдвэрлэлийн бүртгэлийг ашиглан түүхий эд, бүтээгдэхүүний гарц, цэвэршилт, этилийн спиртийн зарцуулалт зэрэгт харьцуулсан тооцоолол хийв. Судалгаагаар ихсийн цусны ийлдсээс ялгасан иммуноглобулины дундаж гарц 14.8 ± 2.1 г/л, цэвэршилт 97.9 ± 1.0%, зарцуулсан спирт 131.3 ± 6.3 л, донорын цусны сийвэнгээс ялгасан иммуноглобулин тунадасны дундаж гарц 25.7 ± 2.4 г/л, цэвэршилт 98.4 ± 0.8%, зарцуулсан спирт 96.9 ± 0.7 л байв. Дээрх дүнгээс үзэхэд донорын цусны сийвэнгээс ялгасан иммуноглобулин нь амаржигчдын ихсийн цусны ийлдсээс ялгасан иммуноглобулинаас гарц 73.6%, уургийн цэвэршилт 0.5%-иар тус тус нэмэгдэж, этилийн спиртийн зарцуулалт 26.1%-иар буурсан буюу иммуноглобулин үйлдвэрлэлийн түүхий эд, технологид хийсэн бидний шинэчлэлт нь бүтээгдэхүүний гарц, чанар, аюулгүй байдалд чухал ач холбогдолтой байна.

Түлхүүр үг: биобэлдмэл, эмчилгээний уураг, эсрэгбие, анагаах ухааны биотехнологи, гаммаглобулин, дархлаа.

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Author Biographies

Janchivmaa Dorjbat, Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia

Department of Biology, National University of Mongolia, Ulaanbaatar 14201, Mongolia

Nergui Zoljargal, Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia

Department of Biology, National University of Mongolia, Ulaanbaatar 14201, Mongolia

Batchuluun Enkhdelger, Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia

Department of Biology, National University of Mongolia, Ulaanbaatar 14201, Mongolia

Gantulga Anujin, Department of Biotechnology and Innovation, National Center for Public Health, Ulaanbaatar 13381, Mongolia

Department of Biology, National University of Mongolia, Ulaanbaatar 14201, Mongolia

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Published

2022-12-29

How to Cite

Dorjbat, J., Sanchimeg, B., Zoljargal, N., Enkhdelger, B., Nomin, B., Anujin, G., Nandintsetseg, D., Uranzaya, E., Zolboo, B., Altantuul, A., Yesukhei, E., & Ichinkhorloo, B. (2022). Comparative study of technology and results of immunoglobulin extraction from placental blood serum and donor blood plasma. Bulletin of the Institute of Chemistry and Chemical Technology, 10(10), 107–111. https://doi.org/10.5564/bicct.v10i10.2602

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