PLANT REGENERATION FROM CALLUS CULTURES DERIVED FROM MATURE ZYGOTIC EMBRYOS OF SOPHORA ALOPECUROIDES LINN. IN MONGOLIA

  • Tsolmon M Plant Biotechnology Laboratory, Institute of General and Experimental Biology, Mongolian Academy of Sciences, Ulaanbaatar
  • Ganbat B Plant Biotechnology Laboratory, Institute of General and Experimental Biology, Mongolian Academy of Sciences, Ulaanbaatar
  • Oyunbileg Yu Plant Biotechnology Laboratory, Institute of General and Experimental Biology, Mongolian Academy of Sciences, Ulaanbaatar

Abstract

The aim of this study is to determine the effect of hormones and selection of the most effective medium using callus cultures derived from mature zygotic embryos of Sophora alopecuroides Linn. for plant regeneration. After 8 weeks of culture, the highest callus induction medium (93.3%) was obtained on MS medium supplemented with 0.2 mglL Zeatin and 2.0 mg/L α-naphthaleneacetic acid (NAA). The best callus proliferation was observed on the same medium.
Shoots regenerated at the highest frequency of 50.0% with 5.8 shoots when calli were cultured on MS medium with 2.0 mg/L BA. Therefore, this protocol provides a basis for future studies on genetic improvement and could be applied to large-scale multiplication systems for commercial nurseries of S.alopecuroides L.

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Abstract
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Published
2018-10-18
How to Cite
M, T., B, G., & Yu, O. (2018). PLANT REGENERATION FROM CALLUS CULTURES DERIVED FROM MATURE ZYGOTIC EMBRYOS OF SOPHORA ALOPECUROIDES LINN. IN MONGOLIA. Proceedings of the Mongolian Academy of Sciences, 58(3), 66-71. https://doi.org/10.5564/pmas.v58i3.1037
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Articles