ANTIBACTERIAL ACTIVITY OF BIFIDOBACTERIA ISOLATED FROM INFANT FAECES

  • Bayar E Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Koen-cho kitami Hokkaido 090-8507
  • Demberel Sh Research Center of Probiotic and Production, Laboratory of Young Animal’s Physiology and Pathology Institute of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar 17024
  • Satomi Ishii Department of Food Science and Human Wellness, Rakuno Gakuen University, Ebetsu-shi, Hokkaido 069-8501
  • Kensuke Miyazaki Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Koen-cho kitami Hokkaido 090-8507
  • Takashi Yoshida Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Koen-cho kitami Hokkaido 090-8507
Keywords: Probiotic, clear zone, pathogenic bacteria, organic acid, pH,

Abstract

Antibacterial activity of bifidobacteria isolated from Mongolian infant faeces was elucidated on pathogenic intestinal bacteria for the development of a new antibacterial bifidobacteria, the permission for which was granted by the Mongolian Medical Ethics Committee Approval (MMECA). A total of forty-nine single colonies were obtained from 3 samples by using a BL medium enrichment. Among them, 29 isolates had Gram−positive, catalase−negative properties, and maul−like or Y−shaped morphology, and then, 20 Bifidobacterium breve and 9 Bifidobacterium longum strains were detected by the B. breve and B. longum specific primers. Organic acids produced by the isolated bifidobacteria in their cell-free supernatants were quantitatively analyzed by a spectrophotometric absorbance at 340 nm, suggesting that D−lactic, L−lactic, and acetic acids were produced, and the pH of the supernatants was at 3.86−4.55. The isolated bifidobacteria showed antibacterial activity toward Escherichia coli and Salmonella typhimurium as high as that of a standard bifidobacteria, however, lower activity against Staphylococcus aureus. The antibacterial activity was probably due to the production of organic acids.

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Abstract
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Published
2018-10-18
How to Cite
E, B., Sh, D., Ishii, S., Miyazaki, K., & Yoshida, T. (2018). ANTIBACTERIAL ACTIVITY OF BIFIDOBACTERIA ISOLATED FROM INFANT FAECES. Proceedings of the Mongolian Academy of Sciences, 58(3), 44-52. https://doi.org/10.5564/pmas.v58i3.1034
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Articles