A study on hemostasis and uterine contraction properties Polygonum aviculare ( L.)

Abstract

Background  Polygonum aviculare (L.) distributed at phyto-geographical regions of Mongolia, where Khuvsgul, Khentii, Khangai, Mongolian Daguur, Central Khalkh, East Mongolia, Depression of Great Lakes, Valley of Lakes, and Gobi-Altai. The aim of the study was to determine the vitamin C content in the aerial parts of Polygonum aviculare (L.) by titration method, and to hemostasis and uterine contraction activity.

Methods: A samples of the aerial parts of the Polygonum aviculare (L.) were collected according to standards from Gachuurt village, Ulaanbaatar city, in August 2019. Vitamin C content was determined in the aerial parts of the Polygonum aviculare (L.). The powdered samples of the plant were extracted with distilled water, and the aqueous extract was used in this study. When examining the hemostatic properties, the activated calcification time was determined using a modified version of the method of U.Bergerhof and L.Rock (1984).

Results: Vitamin C content in the aerial parts was determined by titration and found to be 3.76 mg/ g. The bleeding time from the tail of treated mice was 2 times shorter than that of the control. The fibrinogen formation time in the blood of mice in the experimental group was shortened, and this indicator was different compared to the control.

Conclusions: The extract was shown to enhance the function of the blood coagulation system by exhibiting resistance to heparin, shortening the recalcification time, and increasing the fibrin content in the blood. To assess its uterotonic activity, a 120 mg/kg dose of the extract was injected into the femoral vein of experimental mice at approximately 20 days of pregnancy. The mice were observed over a period of 8 to 14 hours. Uterine contractions and miscarriage, accompanied by internal bleeding, were observed—indicating that the extract has a strong uterotonic effect.