Simultaneous analysis of major ingredients of Gardenia fruit by HPLC-MS/TQMS method

Authors

  • M Galaqin College of Chemistry and Environmental Science, Inner Mongolia Normal University, Hohhot 010022, China
  • K Uwai Muroran Institute of Technology, Hokkaido Muroran 050-8585, Japan
  • M Yuguchi Muroran Institute of Technology, Hokkaido Muroran 050-8585, Japan
  • T Iwasa Muroran Institute of Technology, Hokkaido Muroran 050-8585, Japan

DOI:

https://doi.org/10.5564/mjc.v17i43.744

Keywords:

HPLC-MS/TQMS, geniposide, genipingentiobioside, quantity control,

Abstract

An efficient, accurate HPLC-MS/TQMS method was introduced for the quantitative/qualitative simultaneous analysis of main ingredients, namely geniposide and genipingentiobioside, in the Gardenia fruit. The separation was successfully obtained using a C8 (100mm×2.1mm, 5μm, 30°C) column by gradient elution with ultrapure water as mobile phase, where flow rate was set to 0.2 ml/min and detection wavelength at 240 nm. The analytical method was validated and the quantification of active compounds, namely genipingentiobioside and gardenoside, was performed. Linearity, precision, repeatability, stability and recovery were also reported. The quantitative analysis revealed that both main ingredients as geniposide and genipingentiobioside have performed a good linear relationship in 0.1-100 mg/ml concentration range (r=1.00000 and r =0.99998). The average content was measured to be 4.842% with RSD 0.96% for geniposide and 1.1976% with RSD 0.47% for genipingentiobioside in the Gardenia fruit. Accordingly, this method would be feasible for the quantity and quality control of crude drugs.

Downloads

Download data is not yet available.
Abstract
1745
PDF
2398

Downloads

Published

2017-02-03

How to Cite

Galaqin, M., Uwai, K., Yuguchi, M., & Iwasa, T. (2017). Simultaneous analysis of major ingredients of Gardenia fruit by HPLC-MS/TQMS method. Mongolian Journal of Chemistry, 17(43), 34–37. https://doi.org/10.5564/mjc.v17i43.744

Issue

Section

Articles