Hepatoprotective effects of Paeonia anomala against acetaminophen-induced cell damage through activation of anti-oxidant system

  • O Sarangerel Functional Food Center, Korea institute of Science and Technology, Gangneung, Gangwon-do, Korea and School of Pharmacy, Health Sciences University of Mongolia, Zorig Street-3, Ulaanbaatar 210648
  • K Kang Functional Food Center, Korea institute of Science and Technology, Gangneung, Gangwon-do
  • SB Lee Emerging Technology Research Center, Samsung Advanced Institute of Technology, Gyeonggi-do
  • JH Yun Functional Food Center, Korea institute of Science and Technology, Gangneung, Gangwon-do
  • D Batsuren Institute of Chemistry and Chemical Technology, Peace ave., Ulaanbaatar 13330
  • J Tunsag Institute of Chemistry and Chemical Technology, Peace ave., Ulaanbaatar 13330
  • CW Nho Functional Food Center, Korea institute of Science and Technology, Gangneung, Gangwon-do
Keywords: Paeonia anomala, acetaminophen, hepatoprotection, anti-oxidation, detoxification

Abstract

Overdose of the analgesic and anti-pyretic acetaminophen causes a potentially fatal hepatic necrosis due to a high toxicity and depletion of cellular defense mechanisms. In the present work, the potential hepatoprotective effect of the fruit extract of Paeonia anomala against acetaminophen induced cell damages was evaluated in cultured HepG2 cells and compared to the root extract. The fruit extract showed a potent protection against acetaminophen induced cell death, while the root extract showed a weak protection. Particularly, the pre-treatment of lower doses of the fruit extract, 10 μg/ mL and 20 μg/mL, significantly enhanced cell viability. The level of total glutathione in HepG2 cells treated with the fruit extract prior to the treatment of 40 mM acetaminophen was enhanced, however, the root extract failed for this activity. In addition, activities of quinone reductase, glutathione peroxidase and glutathione reductase were increased and protein levels of glutathione peroxidase 1 and superoxide dismutase 1 were enhanced in the cells treated with 10-20 μg/mL of the fruit extract. Furthermore, the protein level of Nrf2, a crucial regulator for detoxifying and antioxidant systems, was increased by the fruit extract treatment. These results suggest that the fruit extract of P. anomala exerts protective effects against acetaminophen-induced toxicity through activation of key antioxidant systems.

DOI: http://dx.doi.org/10.5564/mjc.v14i0.190

Mongolian Journal of Chemistry 14 (40), 2013, p.5-11

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Abstract
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Published
2014-10-01
How to Cite
Sarangerel, O., Kang, K., Lee, S., Yun, J., Batsuren, D., Tunsag, J., & Nho, C. (2014). Hepatoprotective effects of Paeonia anomala against acetaminophen-induced cell damage through activation of anti-oxidant system. Mongolian Journal of Chemistry, 14, 5-11. https://doi.org/10.5564/mjc.v14i0.190
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Articles