Mongolian Journal of Agricultural Sciences 2020-09-19T18:05:25+00:00 Dr. Bayarmaa Bold Open Journal Systems <p>Scientific journal published by the <a title="MULS" href="" target="_blank" rel="noopener">Mongolian University of Life Sciences (MULS)</a> and <a href="">Mongolian Academy of Agricultural Sciences</a> (MAAS)<strong>.</strong></p> Sequencing the exon 1 of MSTN in Mongolian horse (Equus caballus) 2020-09-19T18:05:25+00:00 Sergelen B Khaliunaa T Myagmarsuren P <p>Myostatin (MSTN) is a protein responsible for muscular tissue differentiation, development, and growth in mammals. Studies on cattle, mice, pig, and dog have provided sample demonstrations of mutations on myostatin, which strongly affected animal Ankole93200phenotype, particularly muscle development. In horse, myostatin gene is usually studied with respect to racing performance. Most Mongolian native horses do race for long distance and such racing is a very popular sport in Mongolia. On the other hand, the MSTN gene also affects meat yield and quality. We partially sequenced of MSTN gene to explore its polymorphic features in Mongolian horse. A 238 bp long segment of exon 1 was sequenced, using 3130 Genetic Analyser and MEGAX. The sequence was registered with accession number LC216412 in the Gene bank database of NCBI and aligned to four reference sequences (GQ183900.1, KC708233.1, AY840554.2, NM 001081817) from this database. The nucleotide diversity was 0.0084, Tajima test statistic was -1.12. so that there were 233 invariable (monomorphic) sites and 5 variable (polymorphic) sites, in the group of animals compared. The Mongolian horse differed from the reference sequence at 3 sites. There was an amino acid difference in the partial sequence, in which isoleucine in the reference sequence was converted into valine. The results of the study indicate that the Mongolian horse seems to differ substantially from the reference genome of equus caballus. Sequencing of the full length of exon 1 or even the whole MSTN gene, including introns, is strongly advised, to get more insights with regard to the identification and characterization of variants at the myostatin locus in Mongolian horses.</p> 2019-10-28T00:00:00+00:00 ##submission.copyrightStatement## Process optimization for amylase production of Bacillus subtilis M4 mutant strain 2020-09-19T18:05:25+00:00 Naramchimeg B Altantsetseg Kh Urantulkhuur B <p>There are many factors that influence the character of bacterial metabolism and enzyme production.For the<br>maximum production of the desired products, the media components and fermentation conditions should be<br>optimized. In our investigation, we improved the amylase production of Bacillus subtilis M4 mutant strain by<br>the combination of two optimization techniques. The cultural conditions (time period, temperature, pH,<br>inoculum volume) and medium ingredients (various carbon, organic and inorganic nitrogen sources, chlorides, sulfates, phosphates, carbonates) were optimized by one factor at a time methodology (OFAT) and response surface methodology (RSM) to increase the amylase production. The optimum conditions for amylase production were found be the following: 35ºC, pH range 7 and incubation time 72h, inoculum volume 8% (v/v). Optimum medium composition for amylase production was the following: starch 12.9 g, peptone 9.75<br>g, calcium carbonate 0.439 g, magnesium sulfate 0.464 g and potassium chloride 0.464 g per liter. When<br>applied to our optimized medium in the fermentation process, the enzyme activity increased from 0.741 to<br>1.58 U/ml, which means a 2.1-fold increase compared to the original medium.</p> 2019-10-28T00:00:00+00:00 ##submission.copyrightStatement## Morphological and molecular identification of Beauveria bassiana from agricultural soils 2020-09-19T18:05:24+00:00 Norjmaa U Nasamdulam D Enkhjargal B Banzragch D <p>The soil environment is an important reservoir for a wide variety of entomopathogenic fungi, which can significantly contribute to the control of insect populations, especially on agricultural pests. Comparison of entomopathogens with conventional chemical pesticides, there are advantages in using biological control agents, such as human safety and other non-target organisms; pesticide residues are minimized in food and biodiversity increased in managed ecosystems. The main objective of this study was to detect and identify the entomopathogenic fungi from agriculture soils based on morphological and molecular identification. Total of 115 soil samples were collected in spring (before sowing) from different crop fields in “Unjin” and “Nart” Research and Education Centers of the university, which are located in Bornuur sum, Tuv Province from 2016 to 2018. Isolation of entomopathogenic fungus, <em>Вeauveria bassiana</em> was isolated using Dilution plate method in Peptone Dextrose Agar Yeast. Based on microscopic observation, hyphae branched and formed conidiogene cells, and single cell <em>В. bassiana conidium</em> was round and tend to oval with hyaline color. Colonies on PDAY were white to pale yellow and sometimes red pigments in reverse. Using PCR method with primer specific for identification <em>В. bassiana</em>, molecular analysis confirmed that all six isolates has same size of band which, appeared on agarose gel.</p> 2019-10-28T00:00:00+00:00 ##submission.copyrightStatement## Suitability assessment for siting water harvesting ponds 2020-09-19T18:05:23+00:00 Myagmartseren P Ganpurev D Myagmarjav I <p>This paper represents test results of methodology for identifying suitable sites for the water harvesting pond<br>using the "Multi-Criteria Analysis" in Geographical Information System. In order to do this, the objectives of<br>the identification of harvesting sites were (i)to estimate constraint map (ii) to identify the multi-factor map and<br>(iii) to measure the overall value of the suitability. According to the results of the assessment of the water<br>harvesting suitability, 10.23%or 173736 hectares area of Erdenetsagaan soum of Sukhbaatar aimag(province)<br>are suitable, and 22.71% or 385534 ha area is moderately suitable, and 33.29%or 565198 ha land is limited for<br>pond construction and 33.78% or 573532 ha territory is unsuitable for water harvesting.</p> 2019-10-28T00:00:00+00:00 ##submission.copyrightStatement## Mongolian crude propolis content analysis and antioxidant activity 2020-09-19T18:05:23+00:00 Ichinkhorloo P Gantsetseg G Selenge Kh Undrakhbayar Ts Otgonsugar P Bat-Erdem B Munkhjargal B Byambajav Ts Undarmaa T <p>Propolis, a precious bee product has been widely used for pharmaceutical purposes for centuries. Chemical components, properties, and activities are differed based on geographic origins, respective plant sources, and bee species. In our research, Mongolian propolis from 2 different places was examined for chemical content and antioxidant effect. The samples were collected from Northern and Central parts of Mongolia and were examined for a total of 8 contents. Crude Mongolian propolis sample from central part of Mongolia displayed higher results in every aspect with total moisture of 2.1%; ash 1.7%; fat 67.8, 69.3%; and 1.05% of protein. The free amino acid in 70% EtOH was higher in sample from northern part with 1.21µg/mg and the total amino acid was quantified by HPLC and highest concentration detected in methionine 1.90mg/g, and 2.62 mg/g. Totally 3 alkaloids and 2 phenolic were detected by TLC and high antioxidant activity was presented in DPPH radical scavenging test. The results suggest that the best solvent for propolis samples is EtOH 70% solution. The test propolis samples contain different types of alkaloids and phenolic, have high amount of methionine and has high antioxidant effect.&nbsp;</p> 2019-10-28T00:00:00+00:00 ##submission.copyrightStatement## Some results of evaluating the level of sustainable development of agricultural production in Mongolia 2020-09-19T18:05:22+00:00 Solongo Kh Guo Xiao Chuan Batkhishig I <p>In this research work, we evaluated the level of the sustainable agricultural development of Mongolia by a criteria consisting of 3 sub-systems and 14 indicators with a multi-index comprehensive evaluation method by using the data of 2005-2017. According to the research, the sustainable agricultural development level has reached 55.53%, where the economic sub-system has the highest impact and the social sub-system has the lowest impact but the development tendency of the environmental sub-system is at the same level as the general level and the level of sustainable agricultural development strongly depends on the environmental and climatic conditions.&nbsp;</p> 2019-10-28T00:00:00+00:00 ##submission.copyrightStatement##